FastQCFastQC Report
Sun 14 Apr 2019
SRR6575752_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575752_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167549
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16240.13909480458635998No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13180.11288605446109756No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12810.10971702258320636No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11860.10158031911294514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15950.051.3304061
TCAACGC21550.037.288654
ATCAACG21650.036.682313
CAACGCA22200.036.4085435
AACGCAG23400.034.5414356
ACCTAAG5800.034.072771
GTACATG45800.031.1288641
TAAGACA9300.030.8229874
TACATGG46350.030.5198042
ACATGGG46750.029.9545273
CTAAGAC8250.029.6194653
TATCAAC29200.027.3609432
ACGCAGA31000.025.7700377
TAGGTAT3650.025.749275
CATGGGG28700.025.0517084
CGCAGAG32450.024.6354058
CCTAAGA8600.024.5911642
TTAGGAC1151.3787349E-424.5177843
GGTATCA6950.023.6956921
AGGTATA3650.023.1743436
Produced by FastQC (version 0.11.2)