FastQCFastQC Report
Sun 14 Apr 2019
SRR6575753_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575753_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823292
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13080.1588743726405698No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA12840.1559592465370731No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12820.1557163193617817No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10960.13312409205968234No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10920.13263823770909958No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10520.12777969420327173No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG10090.12255675993450682No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9920.12049187894453No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9800.11903431589278166No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8430.10239380438532136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10100.059.5734021
TCAACGC15000.038.8521544
ATCAACG15250.038.5234223
CAACGCA15850.037.065125
AACGCAG17000.034.5577746
GTACATG28900.031.392311
TACATGG29000.030.9598732
ACGCAGA19600.029.7337937
ACATGGG30500.029.4318943
ACCTAAG4700.029.0044041
TCTATAC650.00615407928.92223
GGTATCA3650.028.3330691
TATCAAC21300.028.248422
GGGTACC4550.027.8892637
GGTACCT4450.027.459848
CTAAGAC5750.026.9730953
ATACGGT700.00883590126.8563276
TAAGACA6850.026.758314
CTAATAG909.5170626E-426.1103173
TGGGTAC4900.025.8971756