FastQCFastQC Report
Sun 14 Apr 2019
SRR6575754_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575754_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769398
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12270.15947533006324424No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA12260.15934535831910143No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11560.1502473362291038No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10630.13815996402382122No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9830.12776222449239535No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9370.12178352426182547No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG9120.11853423065825489No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8940.11619473926368408No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8870.1152849370546843No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8260.10735666066197208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10400.055.1407171
AGGTATA1700.038.695076
CATATAG801.160371E-535.2401543
GTCTTAG2550.035.0234831
ATCAACG16300.034.30333
TCAACGC16600.033.683364
CTTAGGT2150.032.781543
GGTATCA3750.032.5902751
CAACGCA17750.031.7657725
GTACATG29600.031.4426691
GTTAATA600.004157881331.3368021
ACCTAAG4550.030.992441
TACATGG29900.030.8127732
AACGCAG18350.030.471056
ACATGGG31050.029.3573363
CTAAGAC5550.028.7847523
TATCAAC20100.028.7643762
TAGGTAT2350.027.9921785
TAAGACA6300.027.5954654
TTAGGTA2450.026.849644