FastQCFastQC Report
Sun 14 Apr 2019
SRR6575755_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575755_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189160
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16290.1369874533283999No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15810.1329509906152242No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15620.13135322412459216No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13840.11638467489656565No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12970.1090685862289347No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12480.10494803054256786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17450.057.6520651
TCAACGC24400.040.4498064
ATCAACG24900.039.637563
CAACGCA26200.037.8501975
AACGCAG27100.036.7666056
TATCAAC31850.031.2912542
ACGCAGA34350.029.1433757
GTACATG50550.028.5505031
TACATGG50150.028.3095252
ACATGGG52100.027.2430783
CGCAGAG36950.027.0926918
GTCTTAG5300.025.7228261
TTAGGTA3350.025.25314
GTACCGT1151.3774814E-424.5211266
ACCTAAG4800.024.4847281
AGAGTAC35450.024.39431210-11
GCAGAGT41600.024.2902589
AGGTATA2602.7284841E-1123.4994136
TACCGTG1252.4239131E-422.5594337
CATGGGG31350.022.4874744
Produced by FastQC (version 0.11.2)