FastQCFastQC Report
Sun 14 Apr 2019
SRR6575756_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575756_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048463
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14890.14201741024719042No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14510.13839305726573087No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13910.13267039466342637No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA12890.1229418682395087No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11030.10520161417236469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13900.048.028161
GTACATG46850.032.8141021
TACATGG46750.032.180352
AGGTATA2650.031.915416
ACATGGG47400.031.4235123
TCAACGC20700.031.3243834
ATCAACG20750.031.2489033
CAACGCA21350.030.5907925
ACCTAAG4800.030.3628751
TAGGTAT2850.029.6757325
AACGCAG22200.029.4195216
TTAGGTA3050.027.7297824
GTCTTAG5450.026.7416111
GTATTAC2550.025.8113231
CATGGGG26800.025.7724884
ATATACT3100.025.7668324
TAAGACA7400.025.3981484
TATAAGC1501.1594657E-625.0738562
ATGGGAG11450.025.0321485
CTAAGAC7350.024.9316523
Produced by FastQC (version 0.11.2)