FastQCFastQC Report
Sun 14 Apr 2019
SRR6575756_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575756_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048463
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13520.12895066397192845No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13220.12608933267077616No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12940.12341875678970073No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12460.11884062670785712No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10590.10100499493067472No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC10510.10024197325036745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15400.050.407891
TCAACGC20850.036.287564
ATCAACG21400.035.354933
CAACGCA21950.034.254955
GTACATG42800.033.5267451
TACATGG43500.032.412412
AACGCAG23600.032.0576066
ACATGGG44550.031.3290083
TAGGTAT2950.028.6740075
TATCAAC27100.028.0947462
CTTAGGT3050.027.7338773
AGGTATA2950.027.0797166
ACGCAGA27700.026.8036867
GTCTTAG4600.025.5692211
GGTATAG3150.025.360377
CGCAGAG29600.025.2575938
CATGGGG27700.024.9387954
ACCTAAG5500.024.8067931
TAATACT2751.8189894E-1223.9239714
GGTATCA6900.023.8646051
Produced by FastQC (version 0.11.2)