FastQCFastQC Report
Sun 14 Apr 2019
SRR6575758_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575758_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014413
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12070.11898506821186243No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11870.1170134846458001No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11650.11484474272313151No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10610.10459250817960733No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10610.10459250817960733No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10530.10380387475318238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13250.059.3200531
ATCAACG17400.044.56273
TCAACGC17900.043.3179364
CAACGCA19050.041.1963125
AACGCAG20000.039.7094156
TAGGTAT2700.038.2909135
GGTATCA4850.036.8759161
TTAGGCG550.00271873134.1770174
CTTAGGT3050.033.8968733
TTAGGTA2950.033.4529274
TATCAAC23450.033.2693942
ACGCAGA24850.031.5810747
GTACATG37250.030.829361
TACATGG37550.030.5393312
CGCAGAG26500.029.4577038
GTCTTAG5050.028.891581
ACATGGG39350.028.661793
AGGTATA3450.028.6046736
GGTATAG3650.028.3247877
TATACCG1205.848682E-627.4128154
Produced by FastQC (version 0.11.2)