FastQCFastQC Report
Sun 14 Apr 2019
SRR6575760_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575760_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641098
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20280.31633229241083266No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18310.28560376104745294No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC9540.14880720264296565No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8790.13710852318990233No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8690.1355486992628272No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA8580.1338328929430446No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8070.1258777909149615No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG7830.12213421348998126No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG7510.11714277692334091No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT7250.1130872347129456No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA7240.11293125232023808No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA7110.11090348121504046No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6970.1087197277171353No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC6900.10762785096818271No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT6860.10700392139735267No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA6730.10497615029215503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATATA801.1572914E-535.2524871
TATTCGC902.5922805E-531.3331019
TAATAAT3100.030.3223533
GTATAAG3950.029.7489341
ATAAGAC953.749906E-529.6839893
CTATTGA3150.028.3489959
ATAAGGT3500.026.8569413
ACCTAAG1956.039045E-1026.5146921
GTATAAT1258.01229E-626.3218561
CCTAAGA2156.548362E-1126.234412
GTCCTAC3050.026.198571
CTTAAGA909.509152E-426.1129532
GTTAATA3850.025.638171
GTACTAG3850.025.638171
CCTTAAT1851.0011718E-825.4071981
CATGGGG18950.025.2979664
TAAGGTG4000.024.6748144
GGGCTAT3250.024.5844336
TTAATAA4050.024.372092
TAGGCAT4650.024.2578855
Produced by FastQC (version 0.11.2)