FastQCFastQC Report
Sun 14 Apr 2019
SRR6575760_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575760_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641098
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19380.30229387706715666No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19360.30198191228174165No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC8360.13040128030347933No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC8210.12806154441286666No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA7860.12260216066810378No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC7710.12026242477749113No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7620.11885858324312351No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7430.1158949177816808No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG7400.11542697060355828No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA7040.10981160446608786No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6930.10809579814630525No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT6930.10809579814630525No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT6740.10513213268486252No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA6620.1032603439723724No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG6510.10154453765258978No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTCC301.3708863E-462.6649553
TTACGTA301.3708863E-462.6649556
ACGTATA450.001013756341.7766348
TACGTAT500.001701694637.5989727
CGTTAAC1204.098183E-935.3399961
ACGTTAA801.158068E-535.2490358
GTATTAA851.7232398E-533.2611731
TATATAC753.2744632E-431.3324783
TAACCAC1201.6507511E-731.3324785
TATAAGA953.7504167E-529.6833972
GTACTAG4100.028.7317051
CATGGGG18900.028.5971014
GTATAAG4850.028.1748451
TCTTAAT3550.027.8020552
GGGCTAT3900.027.7171886
CTCTTAA3450.027.3159391
CTATTGA3700.026.6749469
ATGGGGG11650.026.2224585
AGGGCTA3950.026.17655
TTTACGT909.514589E-426.1103975
Produced by FastQC (version 0.11.2)