FastQCFastQC Report
Sun 14 Apr 2019
SRR6575761_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575761_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592961
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21330.35972011650007335No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20640.3480836007764423No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG8510.14351702725811646No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC8170.1377830919740084No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC8070.136096640419859No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA7470.12597793109496241No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7160.12074993127709917No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA6770.11417277021591639No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC6710.11316089928342674No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6690.11282360897259686No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG6620.11164309288469224No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6600.11130580257386236No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA6590.11113715741844742No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT6490.109450705864298No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC6270.10574051244516924No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6150.10371677058018992No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6130.10337948026936004No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA5930.10000657716106118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCTC1550.042.442555
TATAGTG608.830015E-539.1583025
ATAATGC801.1593993E-535.2424773
TTAGGTA851.7559192E-533.1693884
GACGTTA1501.0695658E-931.3292857
CTTAGGT902.5956082E-531.3266433
CATGGGG19150.031.1630614
ACGTTAA1602.1173037E-929.3910338
TATATAC650.00615821828.9169033
CGTTAAC1652.9413059E-928.5003979
ATAAGGT3050.027.7317833
ACCTAAG1554.9943992E-827.3352071
GTACATA700.00876212426.9013141
GATCTAT700.00876212426.9013141
AGGTATA1057.426401E-526.851416
AAAACGC1057.426401E-526.851414
ATGGGGG11700.026.5071585
GGGTACC3050.026.1933367
AACGCAA1101.0189995E-425.6308926
TCAAGAC1851.00390025E-825.399983
Produced by FastQC (version 0.11.2)