FastQCFastQC Report
Sun 14 Apr 2019
SRR6575762_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575762_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722625
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA24590.3402871475523266No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20910.28936170212765955No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC10600.14668742432105172No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10420.14419650579484516No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA10340.14308943089430895No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC10080.13949143746756618No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG10020.13866113129216398No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9880.13672375021622557No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC9620.1331257567894828No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT9210.12745199792423456No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT8860.12260854523438852No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG8650.11970247362048088No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8510.11776509254454247No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8440.11679640200657326No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA8390.1161044801937381No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT7940.10987718387822176No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC7280.10074381594879778No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTC250.005235636656.3989648
GCATATA1003.3163815E-837.609721
CTATTGA4150.031.7102589
AGTACTC902.5929281E-531.3327563
GGGCTAT4900.029.7341466
GGCTATT4950.029.4338027
CATGGGG21100.028.511324
ATTTAGC1005.3215022E-528.1994823
AGGGCTA5000.028.1994825
CGCTTTA505.325135E-528.19948226-27
TAATAAT4600.027.5864493
TTTAGGG4700.027.0069792
ATAAGCG700.00883468226.856653
GAACCGT1406.3638436E-726.856656
GTACTAT909.499905E-426.1178591
CGTTAAC909.5152686E-426.110639
GTCTTAT1659.284486E-825.6429881
ATTTAGG5400.025.2472631
CTAACTG2800.025.1781084
AACCGTG1501.1617176E-625.0662067
Produced by FastQC (version 0.11.2)