FastQCFastQC Report
Sun 14 Apr 2019
SRR6575762_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575762_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722625
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21960.3038920601971977No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21220.29365161736723755No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC10470.14488842760768034No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC9640.13340252551461684No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA9620.1331257567894828No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA9470.13104999135097734No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC9410.13021968517557517No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT9130.12634492302369832No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8540.11818024563224355No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG8530.11804186126967652No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8200.11347517730496454No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG8180.11319840857983048No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT8010.11084587441619097No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA7990.11056910569105692No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7680.10627919045147899No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7270.10060543158623075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATATA857.743438E-944.349531
CATGGGG21300.031.5544764
GTACTAC600.00410778231.414251
ATGGGGG13350.031.3338185
CGTTAAC1602.073648E-929.450861
ATAACCA2750.029.0549934
ACGTTAA1154.1953117E-628.6071598
GGCTATT4600.027.5854747
GGGCTAT4850.027.134446
TGGGGGG5900.027.0851656
TATTAGT700.00883322126.857562
AGGGCTA4550.026.8575575
GACGTTA1057.420537E-526.85577
TAACCAC2500.026.3204085
CTATTGA4500.026.1097099
TATTCTC2550.025.804325
GTATATA1301.0655662E-525.3730491
GTACTAG3750.025.13141
GTCTAGG950.001284658824.8007241
ACCTAAG1901.29512046E-824.8007241
Produced by FastQC (version 0.11.2)