FastQCFastQC Report
Sun 14 Apr 2019
SRR6575763_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575763_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680970
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA25930.3807803574313112No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25080.3682981629146658No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC11090.16285592610540844No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA9620.1412690720589747No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC9590.14082852401721074No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG9000.13216441252918631No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8960.1315770151401677No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA8900.1306959190566398No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG8400.12335345169390721No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8330.12232550626312466No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8230.12085701279057814No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA8110.11909482062352232No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT8100.11894797127626767No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG7910.1161578336784293No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT7850.1152767375949014No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7650.11233975064980836No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7570.11116495587177115No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC7180.10543783132883976No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7000.10279454307825601No Hit
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG6840.10044495352218161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATA959.3291237E-734.68541
TAGGTAT1107.697781E-834.177125
ACCGTGC753.2641116E-431.3497438
GTATAAG3800.030.9691051
GATATAC804.782709E-429.3709623
TGATCGC3900.026.5267059
CTTAGGT1606.916525E-826.4338653
AGGTATA1458.6521686E-725.927476
TAGAGAG1851.00371835E-825.4019135
TATTCTC2601.8189894E-1225.3042145
CATGGGG18200.025.3042134
GTGATCG4150.024.928718
ACGTTAA1151.3715203E-424.534588
TTAGGTA1751.6463127E-724.1681064
TAAGGTG4350.023.7668484
ATAAGGT4750.023.74413
GTATAAT1602.0094503E-623.5365181
CCGTACT1000.001759352723.496774
GTAGTGT1602.039209E-623.4967696
CTAACGC2853.6379788E-1223.0845453
Produced by FastQC (version 0.11.2)