FastQCFastQC Report
Sun 14 Apr 2019
SRR6575764_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575764_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923962
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18290.1979518638212394No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA17720.19178277894545448No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT17610.1905922537939872No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15980.17295083564042676No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG15060.16299371619179143No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA14830.1605044363296326No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14690.15898922250049244No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA13720.1484909552557356No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13290.14383708420909086No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13100.1417807225838292No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA13000.14069842699158622No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12640.13680216285951152No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG11620.12576274781863325No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT10870.11764553087681094No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10640.11515625101465213No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT10640.11515625101465213No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10130.10963654349421297No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG9730.10530736112524107No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG9700.10498267244756819No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC9550.10335922905920372No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA9530.10314276994075514No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC9530.10314276994075514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19850.054.890861
TCAACGC27900.038.5809484
ATCAACG27850.038.4814343
CAACGCA28650.037.7350435
AACGCAG29150.037.0877846
TAGGTAT3050.035.4461945
GGTATAG3050.033.899557
AGGTATA3150.032.82876
TTAGGTA3300.032.7608764
ACCTAAG6500.032.6548581
GGTATCA8550.031.4454151
ACGCAGA34600.031.1050197
CTTAGGT3500.030.8888263
TATCAAC35650.030.8530372
GTCTTAG4600.030.761821
CGCAGAG37100.029.0089938
CTAAGAC7650.028.8787273
GTACATG51350.028.750971
TACATGG54150.027.3434752
GCAGAGT40050.027.1069439
Produced by FastQC (version 0.11.2)