FastQCFastQC Report
Sun 14 Apr 2019
SRR6575766_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575766_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911728
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA30160.3308004141586087No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22160.24305494621202814No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17110.18766561957074918No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13730.15059315936331888No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11970.13128915641507116No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11660.12788901953214116No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11210.122953336960146No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11170.1225146096204131No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10920.11977256374708245No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10150.1113270624572241No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC9900.10858501658389344No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA9540.10463647052629732No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9440.10353965217696505No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCG156.903016E-493.995679
ACACGAC301.3713224E-462.6637768
GCGTAGT250.005234375656.403591
GTATCAA27750.041.3287541
AGGTATA6600.037.02866
TTAGGTA7000.036.255474
CTTAGGT7000.036.255473
TAGGTAT6950.035.1638455
GGTATAG7200.033.942887
GTCTTAG8550.033.5342941
TCAACGC35250.032.665164
CAACGCA35700.032.385065
ATCAACG35550.032.2573053
AACGCAG36550.031.5033366
ACCTAAG10850.030.7577151
AAGGGTA2600.030.7293535
TCTTAGG9450.030.3405532
GGTAATC2800.030.2128938
CTAAGAC12300.029.421413
TAAGACA13750.028.36964
Produced by FastQC (version 0.11.2)