FastQCFastQC Report
Sun 14 Apr 2019
SRR6575766_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575766_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911728
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA30490.33441991471140514No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23740.2603846761314778No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC19430.21311180527525753No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13650.14971570468385306No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13450.14752206798518858No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13060.14324447642279275No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG12280.13468929329800117No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11930.13085042907533825No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11640.12766965586227472No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11240.12328238246494569No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10710.11746924521348473No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC10470.1148368811750873No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9990.10957215309829248No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9750.10693978905989507No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA9550.10474615236123054No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9540.10463647052629732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGTA2350.058.0023045
GTATCAA23900.043.3890651
GGTAATC3250.041.9378248
AGGGTAA3950.035.697626
TCAACGC32450.032.0105174
CAACGCA33000.031.7618665
ATCAACG32850.031.4776553
GTGCAAG4350.031.42421
CAAGGGT4700.031.001234
AACGCAG34000.030.6894536
TAGGTAT5850.028.9242235
CTTAGGT5900.028.6791023
GGTATCA12350.028.2436161
TATCAAC37950.027.8667162
AGGTATA5950.027.6481556
GTCTTAG8050.027.5205751
GTACATG57100.027.1590981
ACCAGAT11550.026.85673794
ACATGGG57750.026.6140423
TACATGG59400.026.2704032
Produced by FastQC (version 0.11.2)