FastQCFastQC Report
Sun 14 Apr 2019
SRR6575767_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575767_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802708
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA28200.35131081289833915No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21450.26722045874714095No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17320.21576961983685228No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12840.1599585403409459No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT12280.1529821554039576No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11660.145258300652292No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10840.135042879851702No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10650.13267589210522382No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10630.13242673550033138No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10430.12993516945140698No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC9950.12395541093398844No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9880.12308336281686492No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9550.11897227883613966No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9140.11386456843584467No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8910.11099926747958162No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA8520.10614071368417906No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG8430.10501950896216307No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8120.10115758158633027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24250.039.6078641
TAGGTAT5900.033.451915
CTTAGGT5800.033.218463
AGGTATA5850.032.936596
TTAGGTA5850.032.934544
GACTAGA753.241005E-431.3885881
TCAACGC30850.031.0741044
CAACGCA30750.031.0223375
TGGGTAC4750.030.6703516
GGGTACC4600.030.6488427
GGTAATC1859.094947E-1230.5040768
AACGCAG31800.030.4432336
ATCAACG31600.030.3365843
GTCGACC552.973371E-629.90397830-31
GGTATAG6650.029.6809847
AAGGGTA1952.0008883E-1128.918135
GTACATG50750.028.2961181
ACCAGAT10200.028.12230594
ACCTAAG10100.027.970031
ATGGGAG9000.027.6730465
Produced by FastQC (version 0.11.2)