FastQCFastQC Report
Sun 14 Apr 2019
SRR6575768_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575768_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532636
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21470.4030895395729917No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18760.3522105152486877No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10880.20426707920606194No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA9970.18718224077981963No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC9380.17610525762434384No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC9010.16915867496752002No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT8450.15864492824367862No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG8230.15451452774502664No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT8090.15188609106406625No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG7920.14869441795147154No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA7680.14418852649839667No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA7250.13611547097830415No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG6740.12654045164051997No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC6660.12503848782282834No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC6580.12353652400513672No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6330.11884288707485037No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA6280.1179041596887931No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC6270.11771641421158163No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT5840.10964335869148913No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5630.10570070367004859No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC5550.10419873985235695No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA5510.10344775794351115No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT5380.10100706673976224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAAGG1204.1982275E-935.249083
TATTAAG1800.033.9531252
GTGCAAG902.5868061E-531.3413471
CATGGGG15550.031.1310234
ACCTAAG3650.030.9120141
GCCTATT650.00614356228.9304751
GTACTAG3450.028.616011
CGTTAAC2151.8189894E-1228.4258731
TAACAGT2253.6379788E-1227.154854
TTAAGGT1406.358987E-726.8564454
GGTAATC1257.997447E-626.3267331
ATGGGGG10800.026.1104345
TACGGGC4400.025.6356984
TTAACAG2409.094947E-1225.457673
GACGTTA1301.0866181E-525.3070357
CCTAAGA4500.025.0730762
TCTAACG2301.5279511E-1024.528012
GTATAAG3450.024.528011
ATACGGG4600.024.5211013
CAAGGGT1351.455826E-524.369744
Produced by FastQC (version 0.11.2)