FastQCFastQC Report
Sun 14 Apr 2019
SRR6575768_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575768_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532636
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20340.3818743006480974No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20310.38131106421646305No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10810.20295286086558176No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC8950.16803220210425132No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA8650.1623998377879077No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8540.16033463753858168No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT8120.15244932749570062No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC7620.14306205363512792No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG7470.1402458714769561No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT7350.1379929257504187No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA7260.13630321645551557No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA7090.13311154334292086No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6740.12654045164051997No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA6110.11471248657619838No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC6030.11321052275850675No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6010.11283503180408384No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG5970.11208404989523801No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA5760.1081413948737975No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC5610.10532521271562569No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC5560.10438648532956842No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG5490.10307226698908824No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5350.10044383030812787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAATC1305.456968E-1239.7679988
AGGGTAA1305.456968E-1239.7679986
GTGCAAG1500.037.708581
AAGGGTA1500.037.5988355
GTCTATA959.2274786E-734.7315861
CTTAGGT1800.033.9433943
CAAGGGT1807.2759576E-1231.3323654
ACGTCGT600.00415962531.3323657
TGCAAGG1807.2759576E-1231.3323652
TTAGGTA1950.031.3323634
AGGTATA2000.030.5490536
TAGGTAT2000.030.5490535
TCTTAGG2051.8189894E-1229.8039552
GTACTAG3450.027.3250561
GTAATCA1904.456524E-1027.2096849
GGGCTAT3200.024.9679786
CATGGGG18300.024.9117984
CGTTAAC1151.3491817E-424.592551
GCAAGGG2301.5279511E-1024.5209793
GTATCAA34850.024.3455661
Produced by FastQC (version 0.11.2)