FastQCFastQC Report
Sun 14 Apr 2019
SRR6575769_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575769_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515569
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23750.46065609064936025No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23540.4565829210057238No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10330.2003611543750691No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA8820.1710731250327308No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8690.16855163906286064No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG8440.16370262758234108No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC8420.1633147066638995No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC8160.15827173472415915No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7420.14391866074182116No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT7290.141397174771951No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA7130.13829380742441846No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT7080.13732400512831455No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG6900.13383271686234044No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA6750.13092330997402868No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG6380.12374677298285972No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC6040.11715211736935309No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA6020.11676419645091152No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC6000.11637627553246996No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5800.1124970663480543No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC5580.10822993624519704No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC5370.10415676660156062No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA5320.1031869643054567No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC5310.1029930038462359No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.10221716200935277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG3750.031.3760431
ACCTAAG3650.029.6568091
TAATAGA804.7826106E-429.3693926
ATAAGGT3850.029.2931083
TAAGAGG1452.6589987E-829.1668474
TGATCGC3800.028.4612629
TAAGGTG3850.028.0725614
TAACCAC1205.8436544E-627.4114366
CCTAAGA4200.026.8520162
CTACCCC700.0088398126.8520154
AAGGGTA700.0088398126.8520155
GTGATCG4050.026.7043938
AGGTGAT4050.026.6862626
GTACTAG2305.456968E-1226.6014291
GTGTATG909.4352016E-426.1467021
GTCCTAC2550.025.8390961
CATGGGG17200.025.4079384
CTTCTAC2301.5097612E-1024.5551641
GGGCTAT2900.024.3057066
AGTACTT9100.024.28658112-13
Produced by FastQC (version 0.11.2)