FastQCFastQC Report
Sun 14 Apr 2019
SRR6575770_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575770_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114134
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2430.21290763488531023No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2210.1936320465417842No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1800.15770935917430387No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1670.14631923878949304No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1470.12879597665901482No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1370.12003434559377574No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1330.1165296931676801No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1320.1156535300611562No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1280.11214887763506054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.107768062102441No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1190.10426340967634536No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1150.10075875725024971No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACC250.005216957556.4191974
AACACTC804.5802153E-946.99547
ATGGGGA1300.043.3993875
TATTGTG555.242562E-542.741825
ACACTCT802.4345354E-741.120978
CACTCTA853.9170845E-738.702099
ATGGGAG2150.037.1754465
CATGGGG4150.036.2533044
GTACATG10950.035.763131
TACATGG10950.035.6376952
TTATTGT801.1468592E-535.2624
ACATGGG11100.035.156113
GTATCAA6650.031.2178211
ACACTGC804.7569792E-429.3721248
ATATCAA650.006121081828.9329243
ATGGGGG1802.3101165E-1028.7325
TTTGAAC1154.142006E-628.6184373
GCTTTGA1503.502828E-828.3088871
TGGGGCA1005.2699557E-528.2095996
AACGCAG7350.028.1456346