FastQCFastQC Report
Sun 14 Apr 2019
SRR6575771_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575771_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581777
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21160.3637132440780574No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19950.3429148969450494No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA8370.1438695582671711No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG8080.1388848304419047No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC8030.13802539460996224No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA8010.13768162027718522No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG7760.13338444111747283No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT7440.12788405179304096No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC7180.12341498546694007No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC6830.11739893464334271No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA6090.10467928433059402No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT6020.10347607416587455No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6020.10347607416587455No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG5950.10227286400115508No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5930.1019290896683781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA1201.6478043E-731.3379171
CTTTATA600.004156330631.3379172
TAATAAT2700.029.5918353
TGATATA650.006152474328.9223354
ACCTAAG1503.686E-828.2041241
GACGTTA856.813035E-427.6463497
ACGTTAA1057.4166746E-526.8564558
TAGTACT3000.026.632654
CCTAAGA1807.4705895E-926.1149312
CGTTAGT551.01852405E-425.63570814-15
GTTAATA3750.025.0703331
CATGGGG16850.023.7085914
TCGATAA1600.023.49939766-67
CTAACTG1602.0363768E-623.4993974
GTACTAG3300.022.7912141
CGATAAT1700.022.11707966-67
TTAATAA3900.021.6954842
GTTAGTC653.1602272E-421.69175114-15
TTGTACT1100.003058202621.3630894
ATCCTAT2701.1368684E-920.8919451
Produced by FastQC (version 0.11.2)