FastQCFastQC Report
Sun 14 Apr 2019
SRR6575771_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575771_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581777
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21330.3666353259066618No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19790.34016470228283346No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC9480.16294903373629416No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA8850.15212014225381892No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC8200.14094747643856667No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG7690.13218123095275336No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA7630.13114990795442238No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC6860.11791459614250821No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG6840.11757082180973123No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT6640.1141330784819613No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6500.11172665815252236No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA6330.10880457632391792No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG6060.10416362283142853No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT5970.10261663833393209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAAG1156.184564E-1140.8689652
CTAGAAC608.819469E-539.1660923
ATACTTA600.004159606531.3328726
GTATAAG3900.030.2343181
TTATACC953.749498E-529.6837794
GTATATA650.00604692929.024941
CTATATC650.00615214428.9226533
ATGGGGG9600.028.8849935
CCTAGAA856.812594E-427.6466562
GTGATCG4500.027.1528248
TTAAGGT1057.416117E-526.8567524
TGATCGC4400.026.7018599
ATAAGGT4600.026.5648273
CATGGGG15500.026.380264
AGGTATA1258.0159225E-626.3196146
GGGTACC2156.548362E-1126.2299217
TATGTAT1458.639254E-725.9306553
TTAGGTA1301.0867476E-525.3073224
GTACTAG3200.025.0566881
TAGGTAA1151.3762036E-424.521385
Produced by FastQC (version 0.11.2)