FastQCFastQC Report
Sun 14 Apr 2019
SRR6575772_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575772_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549703
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA25030.4553367909580264No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22800.4147694300376749No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG9070.16499818993165402No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC8270.15044487659699873No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA7980.14516930051318622No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG7650.13916605876264093No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA7510.13661922892907624No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG7510.13661922892907624No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC7460.13570964684566028No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7310.13298090059541243No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT6790.12352124692788652No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6780.12333933051120333No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC6530.11879142009412356No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA5970.10860410075986487No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC5960.10842218434318168No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT5680.10332852467605233No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA5500.10005402917575491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAAG1055.0913513E-835.819442
ACGGTCC550.002719365834.1726238
TTAAGAC702.1869777E-433.56243
TTAAGGT1351.3280442E-831.3249054
ATTATTA2900.030.8016321
CTAACTG1551.5206751E-930.3144234
TAATAAT2400.029.3670983
GCATATA650.00614309628.9310861
GTATAAG3150.028.3570581
CTTGTAC1354.6381865E-727.844363
ATTAACT3300.027.0533284
ACCTAAG1406.342798E-726.864581
AGCACCG2104.9112714E-1126.8499185
GTGATCG3150.026.8499188
GTCTTAT909.495977E-426.118341
CGTTAAC1458.6158616E-725.9382171
TAGAGCA1101.0192517E-425.6294674
ATAAGGT3300.025.6294673
CATGGGG15550.025.0800674
GTCTATA950.001301617624.743691
Produced by FastQC (version 0.11.2)