FastQCFastQC Report
Sun 14 Apr 2019
SRR6575772_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575772_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549703
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24150.43932814628990563No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22650.412040683787427No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC8700.15826728251437597No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG8270.15044487659699873No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA7870.1431682199296711No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7840.14262247067962153No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA7830.14244055426293836No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC7190.13079790359521415No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG7130.129706405095115No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT6630.12061058426095547No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG6550.11915525292748994No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA6050.11005943209333038No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5950.10824026792649849No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC5740.10442002317615148No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA5710.10387427392610192No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC5660.10296469184268597No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAACC405.660487E-447.0229348
GTGTAAC600.004134421231.3714681
TATTAAG902.5925092E-531.3315162
GACGTTA804.7640444E-429.3893349
ATAATAC804.7818464E-429.3706233
TGTATGA650.006153158428.9213982
GTACTAG3350.028.093851
TGTATGT1704.0690793E-927.6454542
GTGTATG1406.2909567E-726.8898281
CTATTAT1057.353514E-526.8898281
ACTACTC700.00883850526.853143
GTCTTAA909.4440393E-426.1428891
ATAATAG909.519614E-426.1072223
TAGGCAT4200.025.734265
CGTTAAC1501.1484444E-625.0971741
ATAGGTA2651.8189894E-1224.84909294
ATGGGGG10650.024.7099325
TGGGGGG5400.024.3667376
ACCTAAG1551.5280457E-624.2875881
GAGTTGC3900.024.12091394
Produced by FastQC (version 0.11.2)