FastQCFastQC Report
Sun 14 Apr 2019
SRR6575775_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575775_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240463
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15920.12833917658164734No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA15480.12479211391230532No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14420.11624691748161775No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13870.11181308914494023No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12590.10149436137958166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15400.057.391881
TCAACGC21250.040.2534644
ATCAACG21450.039.8781363
CAACGCA22750.037.5993845
AACGCAG24200.035.346536
ACCTAAG5450.031.0541271
TATCAAC28300.030.2341862
GTACATG47600.029.6297261
TACATGG47950.029.609542
AGGTATA3500.028.1995416
ACGCAGA31350.027.434967
ACATGGG50950.027.3047543
TAGGTAT3800.025.9732635
CGCAGAG33250.025.7258978
CTAAGAC8100.025.5304493
CTTAGGT3700.025.4049913
TAAGACA9150.025.1689874
GATATAA2151.873559E-924.0529061
TAACACT2552.1827873E-1123.9603964
TTAGGTA3750.023.8129444
Produced by FastQC (version 0.11.2)