FastQCFastQC Report
Sun 14 Apr 2019
SRR6575776_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575776_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128763
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15410.13652112976771918No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA14960.13253446471934321No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14450.12801624433118378No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14130.12518128251900532No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC11300.10010958899255203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14100.053.0015261
TCAACGC19300.037.4920434
ATCAACG20150.036.1436773
CAACGCA20500.035.2973865
AACGCAG21600.033.7173656
GTACATG44550.032.1782531
TACATGG45000.031.5431292
ACATGGG46850.029.8870013
TATCAAC26350.028.1829742
ACGCAGA26900.026.8994927
CATGGGG28650.026.4044384
ACCTAAG5750.026.1572761
CGCAGAG28750.025.1685688
CTAAGAC8450.025.0225453
TAAGACA8350.024.75954
ATAGTAC1000.001761541623.493393
GGTATCA5050.023.2679981
AGAGTAC27200.023.14789810-11
ATCTAAT1903.6386518E-722.26381
GCAGAGT33700.021.8899829
Produced by FastQC (version 0.11.2)