FastQCFastQC Report
Sun 14 Apr 2019
SRR6575776_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575776_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128763
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA15400.13643253721108858No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13830.12252350582008802No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13020.11534750873301126No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12130.10746277119288991No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11850.10498217960723377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14800.053.119011
TCAACGC19550.038.6976744
ATCAACG20000.037.8269773
CAACGCA20700.037.0018125
AACGCAG21400.035.5718966
GTACATG43600.032.931281
TACATGG43200.032.417292
GTACTAA600.004128375631.383691
ACATGGG44700.030.6959483
TATCAAC25750.030.1127852
GGTATCA6050.028.0118851
ACGCAGA28400.026.805367
ATTATCC6850.026.753453
GGTACCT7050.026.0129248
CATGGGG27500.024.9474354
CGCAGAG30600.024.894728
CATGGGT10850.023.8198344
TTAGGTA4000.023.4950164
GGGTACC8000.022.9086557
ATGGGAG12150.022.8182055
Produced by FastQC (version 0.11.2)