FastQCFastQC Report
Sun 14 Apr 2019
SRR6575777_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575777_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1092644
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA23380.21397637290828486No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21220.19420781151042793No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15540.1422238167234708No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14350.13133280373113293No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14040.1284956490860701No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13980.12794652238057408No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12270.11229641127393736No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11570.10588993304315039No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11360.10396798957391427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14750.053.219241
TCAACGC20400.037.3223884
ATCAACG20450.037.2311333
CAACGCA21200.035.9139945
AACGCAG22300.034.1424526
GTACATG46650.032.848091
ACCTAAG8000.032.3159451
TACATGG47350.032.2632062
GGTATCA5400.031.3366761
ACATGGG49150.030.6948873
TAAGACA10450.028.7838084
TATCAAC26850.028.1855012
CTAAGAC10050.027.5911943
CCTAAGA10350.026.3409732
GGTACCT6450.026.2317548
ATTATCC9550.026.082973
ACCAGAT11100.025.82803594
ACGCAGA29950.025.421597
CATGGGG32000.025.1148554
GGGTACC6750.025.0658997