FastQCFastQC Report
Sun 14 Apr 2019
SRR6575777_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575777_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1092644
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA23030.2107731337928914No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18900.17297491223124825No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14270.13060063479047157No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13680.12520088885309397No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12820.11733007274098425No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12520.11458443921350413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15850.049.6635551
TCAACGC21000.037.152274
ATCAACG21400.036.677463
CAACGCA22100.035.3030665
TAGGTAT4700.033.9999055
AACGCAG23400.033.5426376
GGTATCA5900.031.9564531
TTAGGTA5200.031.6345234
GTACATG42800.031.4972651
CTTAGGT5100.031.333243
GTCTTAG6300.030.6756571
TACATGG44650.029.9999122
ACCTAAG9600.029.4598581
TATCAAC26850.029.4077362
ACATGGG45250.028.9789223
CTAAGAC10550.028.9572623
AGGTATA5400.028.722146
TAAGACA10800.027.8517724
GGTATAG6150.027.5095967
TCTTAGG7200.027.4165862
Produced by FastQC (version 0.11.2)