FastQCFastQC Report
Sun 14 Apr 2019
SRR6575778_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575778_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988931
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA23280.23540570575702452No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC19400.1961714214641871No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15730.15906064224905478No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14580.1474319239663839No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13770.139241261523807No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12380.12518568029518742No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11070.11193905338188408No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10670.1078942818053029No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10330.10445622596520891No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9960.1007148122568713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14550.052.982931
TCAACGC20600.036.9510844
ATCAACG20900.036.420693
CAACGCA21400.035.7893035
AACGCAG22000.034.8132326
GTCTTAG5550.033.031381
ACCTAAG8300.032.2813641
CTAAGAC8900.032.2046973
GTACATG42600.032.1098631
TACATGG43700.031.0864772
CCTAAGA9100.030.9930972
AGGTATA4600.030.6438146
CTTAGGT4650.030.3143123
TAAGACA9550.030.0127544
TTAGGTA4700.029.991824
ACATGGG45450.029.2571453
TAGGTAT5000.028.192315
TATCAAC27050.028.1515852
ACGCAGA28000.027.017637
GGTATAG5350.026.3479547