FastQCFastQC Report
Sun 14 Apr 2019
SRR6575779_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575779_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041348
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12630.12128510353887462No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA12180.11696378156005485No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11700.11235437144931376No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11450.10995363701663613No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC10570.10150305181361081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14500.058.0305861
TCAACGC19750.040.6927954
ATCAACG20050.040.3183363
CAACGCA20550.039.3373575
AACGCAG21500.037.5991946
TATCAAC24650.033.3728332
GTACATG41400.032.2470471
TACATGG43050.030.574322
CTTAGGT3550.030.4500523
GTCTTAG5400.029.5976451
ACGCAGA27400.029.5030177
ACATGGG45150.029.354853
TGCGTAT650.00615455128.92245794
TAGGTAT3450.028.6080825
AGGTATA3500.028.1993946
GGTATCA5050.027.9255561
CGCAGAG29500.027.2434848
GTATAGA1101.0170661E-425.640741
GTGTTAA1851.0017175E-825.409741
TTAGGTA4450.025.347774
Produced by FastQC (version 0.11.2)