FastQCFastQC Report
Sun 14 Apr 2019
SRR6575780_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575780_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926923
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA12730.13733611098224988No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC9980.10766805872763972No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9550.10302905419328251No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13500.053.008641
CTAGCGT450.001014793541.7699133
ATCAACG17550.040.4311333
TCAACGC17950.039.7919544
CAACGCA18550.038.5048835
AACGCAG19750.035.929356
CGATACA550.00269416634.240031
ACGCAGA24150.029.5778087
GGTATCA5300.029.3139881
TATCAAC24100.029.2491842
GTACATG35000.028.7860831
CGCAGAG25850.027.6520448
TACATGG35750.027.4732322
ACATGGG36150.027.1677763
ACCTAAG4600.026.6104581
CTAAGAC5600.026.012963
GCAGAGT29900.023.7492549
AGAGTAC24850.023.56064610-11
CATGGGG22950.023.1372554
TATATAC1652.6672715E-622.783593
Produced by FastQC (version 0.11.2)