FastQCFastQC Report
Sun 14 Apr 2019
SRR6575781_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575781_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107571
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.2500673973468686No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2370.2203196028669437No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2160.200797612739493No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1940.18034600403454462No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1470.13665393089215494No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1380.12828736369467605No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1330.1236392708071878No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1320.12270965222969014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.12178003365219252No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1190.11062461072222068No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1150.10690613641223007No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1090.10132842494724416No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC250.00522645556.3910753
GCACCAA500.001696496237.5940486
CATGGGT1405.002221E-1033.5661164
ATGGGAG1658.367351E-1131.3283755
TTAACTG753.259017E-431.3283735
GTATCAA8200.030.4440081
CGGCCCC804.7309953E-429.39768494
AACGCAG8700.028.6276516
AATCATG856.7819847E-427.6426836
GTCCCGC350.00881715826.85913514-15
ACGCAGA9250.026.417447
TTTAACT909.470251E-426.106984
TTTAACC459.501233E-426.1069832-33
TTGAACA909.470251E-426.106984
TAGGCGG909.470251E-426.106985
TGAGCAG909.470251E-426.106985
CAACGCA9750.026.026655
ACATGGG10400.025.7555393
CTCTAGG1101.01104066E-425.6323072
GTACATG10700.025.5319541
Produced by FastQC (version 0.11.2)