FastQCFastQC Report
Sun 14 Apr 2019
SRR6575782_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575782_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596854
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11210.18781812637596462No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10200.17089606503433No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA6680.11192016808130632No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA6530.10940699065433088No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT6500.10890435516893579No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG6470.1084017196835407No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC6260.10488327128577508No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT6230.10438063580038No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC6220.10421309063858163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16850.051.6167761
ACCTAAG4050.035.9853481
CTAAGAC5050.035.3643533
TTCGATA550.00271671534.1798974
ATCAACG26000.033.078913
TCAACGC26000.033.078914
CTTAGGT1850.033.0251733
GTATACA1001.4065881E-632.909181
CAACGCA26650.032.448465
ATGGGAG7600.032.156095
TAAGACA6200.031.8369224
AACGCAG27550.031.7296146
TATCAAC28100.030.6170852
AGGTATA1859.094947E-1230.4847766
TAGGTAT2051.8189894E-1229.8032045
ATAGGGT1602.1300366E-929.373353
GTCTTAG2750.029.0626531
GGTATAG1952.0008883E-1128.9214537
GTACATG43300.028.3381611
TACATGG44850.027.8829172
Produced by FastQC (version 0.11.2)