FastQCFastQC Report
Sun 14 Apr 2019
SRR6575782_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575782_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596854
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10180.17056097471073328No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA9920.16620480050397585No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT6830.11443334550828176No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG6610.11074735194871778No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA6200.10387800031498491No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT6010.10069464224081602No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC5970.10002446159362256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15950.046.3876041
GTACATG44050.032.2020531
ACATGGG43700.031.5178093
TACATGG44600.031.5141942
GGTAATC753.2737033E-431.3332798
CAACGCA24900.029.8257375
TCAACGC24950.029.7659684
ATCAACG25050.029.461973
AACGCAG25650.029.3201436
TATCAAC25500.028.9420532
ACCTAAG4000.028.2757571
TAATAGT1853.274181E-1027.9482364
CATGGGA23850.027.1971994
CATGGGG20300.026.62784
ATTGTAA2900.025.9331596
TTAAGAC1101.016387E-425.6406143
ACGCAGA29800.024.9194187
AATAGTA1351.4549909E-524.3723685
CGCAGAG31000.024.106418
TAGGGTG3900.024.1045425
Produced by FastQC (version 0.11.2)