FastQCFastQC Report
Sun 14 Apr 2019
SRR6575783_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575783_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555062
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10280.18520453570952433No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA9130.16448612947742774No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT6040.10881667273205517No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT5930.10683491213594157No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG5870.1057539518107887No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA5830.10503331159402013No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC5740.10341187110629083No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC5560.10016899013083223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14500.052.8256721
TCAACGC21550.034.4505464
ATCAACG22050.033.882453
CAACGCA21950.033.8227465
AACGCAG22800.033.386166
ATAGACG851.756238E-533.167958
GTACATG38750.032.8641971
TACATGG39800.031.9971752
TATCAAC23550.031.5276492
ACATGGG40800.030.4039553
ACCTAAG3600.030.022771
GTTATAT1102.9686562E-629.9041021
GGTATCA6400.028.635851
TATTGTG1555.086258E-827.2833145
GTATTGA2950.027.080231
ACGCAGA27550.026.9477067
GTACTTG4200.026.8526651
CATGGGG19500.026.7469754
CTAAGAC4500.026.1044063
CGCAGAG28900.025.6889028
Produced by FastQC (version 0.11.2)