FastQCFastQC Report
Sun 14 Apr 2019
SRR6575783_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575783_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555062
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10070.18142117457148932No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9900.17835845365022288No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT6120.11025795316559231No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT6060.10917699284043944No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG6010.10827619256947874No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA5700.10269123088952226No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5690.1025110708353301No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA5660.10197059067275367No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC5650.10179043061856154No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC5650.10179043061856154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16400.047.6471061
TAGTACT704.6360055E-640.2792664
ACCCGTC500.001702607137.5939837
TAAGGGT1001.4111865E-632.8947334
TCAACGC24100.032.563254
GGTAATC1457.385097E-1032.4261328
ATCAACG24400.032.1628843
CAACGCA24550.032.1577875
AACGCAG25050.031.5159136
ATATACC902.5943633E-531.3283183
ATGGGAG6700.030.8607315
GTACATG40800.030.8051551
ACATGGG40250.030.7056433
TACATGG41200.030.5679232
AAGGGTA1401.8902938E-830.209455
TATCAAC26150.030.1901972
ACCTAAG3750.030.1267491
CATGGGG18650.028.7246254
ACGCAGA29250.026.8298937
GTTATAT1606.7993824E-826.4785881
Produced by FastQC (version 0.11.2)