FastQCFastQC Report
Sun 14 Apr 2019
SRR6575786_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575786_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956664
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20560.21491349104805868No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17520.18313639898647802No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC15680.16390289589657395No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14590.1525091359139677No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12850.1343209319050367No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12800.13379828236455016No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC12500.130662385121631No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12020.12564494953296038No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11830.12365888127911157No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG11260.1177006765175652No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11090.11592366807991103No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10650.11132435212362962No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT10110.10567973708637515No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9770.10212572021106678No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG9630.10066230149770453No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA9600.1003487117734126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG5100.032.2639431
GAACCGT1807.2759576E-1231.3322896
TAGGACT4050.027.8509224
GTATCAA39200.026.8646721
AGTACTT11000.025.6355112-13
TAGGCAT6250.025.5671465
CTAGGCA6650.025.442764
TAATAAT3900.025.3068493
TACCCCG950.00130467924.7360175
TTAATAA4250.024.3362312
GTAGGAC4100.024.0723673
CAACGCA42300.023.9991995
ATCAACG43050.023.6902683
GGGCTAT4000.023.4992166
GACGTTA1802.1608139E-723.4992167
TAGGTAT1602.0387997E-623.4992165
CATGGGG26250.023.0963734
ACCTAAG2853.6379788E-1223.0941931
GTGTAGA2253.1668606E-922.9842191
GGCTATT4300.022.9527237
Produced by FastQC (version 0.11.2)