FastQCFastQC Report
Sun 14 Apr 2019
SRR6575786_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575786_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956664
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19230.20101101327111714No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18010.18825836448324595No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC14870.15543597334069223No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14020.14655093115242135No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11500.12020939431190052No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11270.11780520642566251No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11210.11717802697707867No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC10950.11446024936654876No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT10940.11435571945845144No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10770.11257871102079728No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10660.11142888203172692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG3550.030.530681
GGGTACC5300.028.3775337
TAGGCAT5200.027.1155515
CTAACGC3150.026.8587133
TGGGTAC5600.026.8573076
GAACCGT2104.9112714E-1126.8573066
CGTGAAT700.00883728326.8559068
GTATAAG5700.026.4552671
CATGGGT9600.026.4390434
GGTACCT6050.026.4120048
CGTTAAC2156.366463E-1126.3014551
GTAAATA1458.64351E-725.932553
CATGGGG25050.025.706094
GGGCTAT4450.025.348476
TCTAACG3350.025.2552072
TATTAAG1501.1615994E-625.068132
AGGGCTA5100.024.8825055
TAGGTAT1901.3322278E-824.7369925
ATGGGTA6550.024.3970955
ACATGGG55650.024.240413
Produced by FastQC (version 0.11.2)