FastQCFastQC Report
Sun 14 Apr 2019
SRR6575787_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575787_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878206
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23340.2657690792365345No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20480.23320268820755038No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC13410.1526976586359009No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12900.14689036513073242No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC11810.13447869861968603No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11740.13368161911897664No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11550.13151811761705112No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10940.1245721391108692No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT10810.12309184860955175No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10810.12309184860955175No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG9930.11307142060063356No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT9670.11011083959799864No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9270.10555609959394492No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG9270.10555609959394492No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8840.10065975408958719No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA8790.10009041158908047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAAG3350.035.080751
TTAGGTA1859.094947E-1230.4779174
TAGGTAT1551.5224941E-930.3140585
GTACTAG4450.028.5218321
GTTAATA4250.027.6518841
GACGTTA1205.853901E-627.4089627
TCTTAGG2750.027.3502252
TAGTACT5400.026.9738984
AGGTATA1755.5661076E-926.8495946
CGATAAT2750.025.62915866-67
CTAACTG2800.025.1714954
CTAACGC2101.4333636E-924.612133
ACGTTAA1351.4601084E-524.363528
CTGTGCG5800.024.3035139
CCTAAGA4700.024.0041892
GGGTACC4900.023.972857
TAGGCAT6100.023.8785325
TAATAAT4550.023.7515643
TAAGACA5550.023.705054
GTACTGT6750.023.667426
Produced by FastQC (version 0.11.2)