FastQCFastQC Report
Sun 14 Apr 2019
SRR6575787_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575787_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878206
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20690.2355939267096786No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20280.23092531820552353No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC13700.15599984513883985No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12300.14005825512465186No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11110.12650790361259204No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10990.12514148161137592No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10560.12024513610701817No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10120.11523492210255909No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT9840.11204660409972148No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9790.11147726159921477No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC9690.11033857659820134No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA9200.10475902009323554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATA1354.6362402E-727.8493562
CATGGGG22100.025.729264
GTATAAG3700.025.4465941
TAGTACT5550.025.4016824
ACTAAGC950.001300697624.7487148
TAGGTAT950.001305455324.7332155
GTACTGT6950.023.6655966
CTATTAT2803.6379788E-1223.53811
CTAACGC2803.6379788E-1223.4965573
CTTAGGT1000.001759850923.4965553
CTAGTAC6250.023.3085843
TCTAACG2853.6379788E-1223.085652
GGCGTCG4550.022.7361838
TAAGGTG3950.022.6042824
GTATAAT2103.786772E-822.4172381
ATGGGGG13000.022.41215
GTATCAA36350.022.4049031
GGGTACC5050.022.3333597
GTACTAG3600.022.2304271
TAGGACT4450.022.1765234
Produced by FastQC (version 0.11.2)