FastQCFastQC Report
Sun 14 Apr 2019
SRR6575788_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575788_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833887
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA32940.39501755033955444No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22220.2664629620080418No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16810.201586066217605No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16120.19331156379701328No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14480.17364463050749082No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG14060.1686079768601741No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13340.159973713464774No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13300.15949403216502955No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA10970.13155259645491535No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10170.12195897046002636No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC10140.12159920948521803No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT9630.11548327291347629No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA9420.11296494608981791No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG8930.1070888501679484No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG8710.10445060301935395No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG8450.10133267457101502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAAG3950.036.8916051
GTATCAA53700.036.6777531
TCGTACC550.002716981834.1807949
AAGGGTA4150.032.842395
TGATCGC1457.4396667E-1032.412829
ATCAACG63400.030.8381923
CAACGCA63700.030.7667375
TCAACGC65000.030.1514054
TTATCCT6750.029.939844
TAGTACT1751.70985E-1029.5419714
GGTAATC4800.029.3741198
CTGTGCG2400.029.3741199
AACGCAG67700.029.296026
TATCAAC68050.029.0124322
ATGGGAG10700.028.9897845
CTATTAT6400.028.6449181
ACCTAAG12900.028.4228651
GTATAAG2650.028.3816131
AGGTATA3700.027.9451076
AGGGTAA4900.027.8154936
Produced by FastQC (version 0.11.2)