FastQCFastQC Report
Sun 14 Apr 2019
SRR6575789_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575789_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778223
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA33510.4305963714770702No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA24690.31726124774004366No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18840.2420899922001791No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16120.20713857082096004No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13730.17642757924142566No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG13390.17205865156902328No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13350.17154466007815242No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13220.1698741877328221No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC10850.13942019189872312No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA10570.13582225146262702No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA9700.12464293653618565No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT9210.1183465407730175No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9090.1168045663004049No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG8620.1107651662826722No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC8280.10639623861026981No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG8140.10459726839222178No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG8070.10369778328319776No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG8050.1034407875377623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAATC3500.036.2471548
AAGGGTA3600.035.240295
GTAATCA3700.034.287859
GTGCAAG3750.033.8437271
GTATCAA57050.033.5339281
AGGGTAA3850.032.951966
TAGGTAT3600.031.3247035
TAGACCG600.004165776531.3247016
ACCTAAG12350.031.2099151
GTCTAGA1252.3592474E-730.0833111
CTAAGAC12950.029.752423
CCTAAGA13450.029.0050492
GGACCGT650.00616125128.9151086
AGGTATA4250.028.745026
ATGGGAG10050.028.5195065
CATGGGG29900.028.1293724
TAAGACA13900.028.0570164
ATCAACG69200.027.3638483
CAACGCA69500.027.3133375
TCAACGC70150.026.9932754
Produced by FastQC (version 0.11.2)