FastQCFastQC Report
Sun 14 Apr 2019
SRR6575789_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575789_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778223
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA35730.45912289922040345No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22970.2951596136325963No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17590.22602775811046447No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16430.21112200487520932No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14750.1895343622586328No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG13890.17848354520490914No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10910.1401911791350294No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG10820.13903469828056997No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC10660.1369787323170865No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC9820.12618491100879825No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG9680.1243859407907502No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG9310.11963151950019468No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA8860.11384911522789741No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG8840.11359211948246198No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8460.10870920031918871No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA8320.10691023010114067No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA7810.10035683859253709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52700.037.1526871
GGTAATC2950.031.8804478
TCTAACG1052.0664793E-631.3289642
AAGGGTA3100.030.3183545
CAACGCA64450.029.894985
ATCAACG64900.029.8325123
TCAACGC65350.029.6989944
GTACAAT650.00615715628.9190443
TATCAAC68100.028.844732
AACGCAG67500.028.6834096
ACGCCTA1154.198655E-628.6047086
CATGGGG29000.028.3581144
AGGTATA3400.027.6432046
CGATAAT706.3588595E-726.8628966-67
CTAGATC700.00884031126.8533974
TAGGTAT3500.026.8533975
CTAACGC1258.02825E-626.3163283
ACGCAGA74150.025.9842387
AGTACTT23800.025.8827712-13
GTGCAAG3100.025.81041
Produced by FastQC (version 0.11.2)