FastQCFastQC Report
Sun 14 Apr 2019
SRR6575790_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575790_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796621
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23530.1309680784094141No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA23120.12868601669467294No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20920.11644080749362276No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG20820.1158842070753932No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20010.11137574368773381No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19520.10864840163840898No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA18860.10497483887809395No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC18390.10235881691241502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25900.052.090951
GGTATCA10000.043.2481961
ATCAACG33450.039.48153
TCAACGC33450.039.48154
CAACGCA35300.037.4123575
AACGCAG36100.036.4530836
GTACATG66400.032.2832261
TACATGG68000.031.2470972
TATCAAC43500.030.798942
ACATGGG70350.029.9961623
ACGCAGA45850.028.7013387
GTACGAC856.818916E-427.6461413
CGCAGAG50100.026.4542128
TAGGTAT7000.024.8420355
GCAGAGT55300.024.1366339
CATGGGG45050.023.681794
ACCTAAG9600.023.5044561
GTATACG1201.8362461E-423.5044561
CTAAGAC12700.022.944123
TTAGGTA7450.022.7106574
Produced by FastQC (version 0.11.2)