FastQCFastQC Report
Sun 14 Apr 2019
SRR6575790_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575790_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796621
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA24690.13742464326087694No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23790.13241523949681094No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18360.10219183678694616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26650.052.308751
ATCAACG34350.040.2280733
TCAACGC34350.040.090134
GGTATCA10400.039.8500821
CAACGCA35900.038.22835
AACGCAG37850.036.1346366
ACCTAAG10900.034.997611
TATCAAC42850.032.9063032
GTACATG70200.030.2565441
ACGCAGA45100.029.9065047
CTAAGAC14050.029.4384543
TACATGG72550.028.9586812
TAAGACA15000.028.513254
CCTAAGA14200.028.13452
ACATGGG74800.027.7105833
CGCAGAG50950.026.6571668
ACCAGAT14150.025.2458594
GGTACCT10900.025.0070118
GTCTTAG9550.024.6573561
GGGTACC11350.024.0155457
Produced by FastQC (version 0.11.2)