FastQCFastQC Report
Sun 14 Apr 2019
SRR6575791_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575791_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619180
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23050.142356007361751No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA22440.1385886683382947No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19710.12172828221692462No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA18670.1153052779802122No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18630.11505823935572326No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17840.11017922652206674No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA17350.10715300337207723No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16880.1042502995343322No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16840.10400326090984324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27300.046.4921951
TCAACGC33200.037.9304354
ATCAACG33450.037.7874223
CAACGCA34600.036.395685
AACGCAG35400.035.7059146
GGTATCA12300.034.014481
ACCTAAG8950.029.4133111
TATCAAC44400.028.5873032
ACGCAGA44300.028.532497
TAGGTAT6100.028.501195
GTACATG62500.028.130051
CTTAGGT6550.027.9778543
GTACTAG1354.6291098E-727.8570521
TACATGG64550.027.0190472
AGGTATA6200.026.5257366
CGCAGAG48100.026.1806768
ACATGGG66600.026.034143
TTAGGTA6900.025.8776994
GGTAATC3550.023.825138
TAGACTC3750.023.8074825
Produced by FastQC (version 0.11.2)