FastQCFastQC Report
Sun 14 Apr 2019
SRR6575792_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575792_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658889
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13470.20443504141061697No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA8840.13416523875796987No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7990.12126473503124198No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7950.12065765250292539No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6980.10593590119124768No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6750.1024451766534272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14550.053.9521181
TCAACGC20350.038.3382954
CAACGCA20700.037.917115
ATCAACG20800.037.508863
AACGCAG22350.035.9590076
ACATGGG37050.034.377173
GTACATG38050.034.3435441
TACATGG38250.034.041082
ACGCAGA24650.032.413147
CGCAGAG24950.032.2117778
TATCAAC24600.032.1017882
CATGGGG19200.031.5774884
GCAGAGT28900.027.8091289
ATGGGGA11750.027.5994425
GGTATCA6250.027.0755631
ATACGCT4250.026.5406428
TATACGC4250.026.5406427
TACGCTA4300.026.2320299
ATATACG4350.025.9305116
GGTACCT5900.024.6944168
Produced by FastQC (version 0.11.2)