FastQCFastQC Report
Sun 14 Apr 2019
SRR6575797_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575797_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485329
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA28930.19477166338232135No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC24810.16703370095110243No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC20660.13909376306528723No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16650.11209637730092122No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15770.10617176396609775No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA15550.10469061063239189No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15460.10408468426860312No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15350.1033441076017502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24700.050.8167651
ATCAACG30000.040.4193043
TCAACGC30000.040.4193044
CAACGCA30750.039.4334685
AACGCAG31100.038.8385626
GGTATCA9500.034.14431
TATCAAC38000.032.5360532
GTACATG60100.032.3831141
TACATGG62000.031.239082
ACGCAGA38850.031.2118177
ACATGGG63300.030.664563
ACCTAAG13000.030.014251
GTCTTAG8800.028.847211
CGCAGAG42550.028.497758
CTAAGAC15750.026.8566823
TAAGACA16050.026.6475184
CTTAGGT7150.025.6359213
GCAGAGT47700.025.4209469
CATGGGG40400.025.1282794
CCTAAGA16900.025.0350552
Produced by FastQC (version 0.11.2)